Sex determination from bones is of vital importance in anthropological studies and medico-legal cases. The present study focused on measurements of the Humerus and to evaluate the differences in sex present in the morphology through statistical analysis. Method: In our study, 120 dry adult humeri (56 right side & 64 left side) were studied of known sex. Damaged bones were excluded from the study. Each Humerus was measured for 11 parameters; measurements were taken by using a sliding caliper as described in the textbook of anthropology and previous studies. The osteometric data of the Humerus of present study is statistically analyzed and compared with other similar studies. Results: Statistical tests were applied to the metrical data obtained to assess whether the differences between the means of each parameter are statistically significant or not. Length of Humerus, the weight of the Humerus, Mid-shaft circumference, Transverse and Vertical diameter of superior articular surface, Bi-epicondylar width of the Humerus have been found to be more discriminatory parameters for the identification of sex from Humerus.