Background and Objectives: Technological advancement is one of the most significant achievements in healthcare, particularly in the twenty-first century, where it has become an integral part of our daily lives; we are more reliant on technology than we were four decades ago. According to recent advancements in healthcare technology, 3D Printing technology has emerged as a valuable tool and resource for doctors involved in organ transplantation. This paper looks into the applications and effects of 3D printing on human organ transplantation. Methods: To conduct the survey and collect data, structured questionnaires were used, as well as random sampling techniques. Secondary data were also used to draw information and compare effects. Results: This study included a total of 84 doctors and other healthcare providers (n=84 respondents). As a result of the research, the mean was 3.15, and the overall level of our study was moderate, with the highest mean being 3.55. The majority of respondents agreed that 3D printing is truly beneficial to human organ transplantation, and comparison with other secondary data validated the statement. Conclusion: 3D printing is regarded as one of the most effective techniques for organ transplantation. It has been discovered that using this technology, 3D printing has the potential to reduce and eliminate the risk associated with human organs and give humans a second chance at life.