Background: Obesity is one of the common significant health hazards and is associated with autonomic dysfunction. Aims and objectives: The present study was designed to assess the underlying autonomic neuropathy in obese subjects and to compare it with age-matched controls. Material and Methods: Thirty obese subjects in the age group of 21-40 years were recruited for the study. Six non-invasive autonomic function tests were performed out of which four were based mainly on parasympathetic control (Heart rate response to standing (30:15 ratio), The S:L (standing to lying) ratio, The Valsalva ratio, Heart rate response to deep breathing ) and the other two were mainly sympathetic (Isometric Handgrip Exercise Test and Cold Pressor Test). Statistical Analysis: Results were analysed by ANOVA with SPSS version 17.0 using unpaired‘t’ test. Results: Results showed that Heart rate response to standing(30:15 ratio), The S:L (standing to lying) ratio, The Valsalva ratio, Heart rate response to deep breathing, Isometric Handgrip Exercise Test and Cold Pressor Test were significantly lower (p <0.05) in obese subjects as compared to control subjects. Conclusions: Latent autonomic neuropathy may be present in otherwise healthy obese individuals. Early and regular screening of obese individuals is necessary to prevent any future complications.