Introduction: The aim of this study is to analyze the utilization of antibiotics at our neonatal intensive care unit (NICU). Neonatal sepsis is one of the most common causes of admission in NICU and the causative bacteria and their respective sensitivity patterns based on the culture sensitive reports helps in achieving the antibiotic policy. Methods: This study was done after obtaining the approval from Institutional Human Ethical Committee (IHEC) of Sri Padmavati Medical College Hospital and Research Institute. The study was carried out during the period of February 2013 to April 2013 at Department of Pediatrics, Neonatology division, the total number of antibiotics used in neonatal intensive care unit (NICU) during the study period was identified and the percentage of the antibiotic prescriptions, individual and fixed dose drug combinations is evaluated. Results: Ampicillin and Gentamicin were the maximum (50%) empirically administered followed by the fixed dose combination of Piperacillin and Tazobactam was used in nearly 16% of the babies. Conclusion: The study concludes the prescription pattern at our neonatal intensive care unit complies with international studies and standards.