In present study, we aimed to evaluate whether some plasma parameters are altered after intraperitoneal(i.p)injection of brucellosis vaccines and two types of Brucella species (Brucella mellitensis and Brucella abortus) in rat. Brucellosis is known to be as the common zoonotic disease. For this purpose, thirty male rats were assigned into five groups and Brucella mellitensis, Brucella abortusalong with RB 51 and S19 vaccines were injected (i.p) to four groups and control ones received distilled water. After three months, plasma malondialdehyde (MDA), hepcidin (Hep) and homocysteine (Hcy) were measured in infected and vaccines-injected cases. The results indicate increase of MDA, Hep and Hcyin infected group rather than control ones (p≤0.01). In respect of vaccines-injected group, increase of Hepalong with no significant changes in others were revealed rather than control ones (p≤0.01).In conclusion, the altered plasma parameters demonstrated the impact of Brucella species and those vaccines upon these parameters in rat and it seems more investigation needs to detect the possible biochemical marker among them.