Context: The present study evaluated oral hygiene & anomalies of tooth formation and eruption in adolescents. This was a cross-sectional study, with the primary data was collected from two different schools in Ahmedabad, Gujarat, India. Aims: Aim of the study was to evaluate oral health that includes maintenance of oral hygiene and dental anomalies in adolescents of Ahmedabad city. Methods and Material: In this study, total 500 healthy male and female subjects between 14-16 years of age were selected for the study and examined for the oral hygiene in the form of cleaning of teeth after all meal, flossing and regular visit to dentist for check-up. Also they were observed for dental anomalies which were divided into three types viz. dentitional, occlusal and space anomalies. Comparison of outcome parameters was calculated with significance test. Results: Awareness of oral hygiene was found improper in more than half of all adolescents. Occlusal anomalies were found in 48.8% of males & 50.8% of females which were most common among all anomalies. Though all anomalies were more prevalent in female than male subjects, dentitional anomalies were significantly more in female subject. Conclusions: Awareness for oral hygiene in adolescent of Ahmedabad city is found to be less. Females are even less aware than male. Malocclusion is the most common anomaly. All anomalies are more common in females.