Inflammation plays an important role in various diseases with high prevalence within populations such as rheumatoid arthritis, atherosclerosis and asthma. The aim of the present context to investigate the anti inflammatory activity of extract of Nymphaea Alba flower (NAF) in Swiss Albino mice. The anti inflammatory activity was test using acute inflammatory models like acetic acid-induced vascular permeability chronic models like; cotton-pellet induced granuloma. Oral administration of ethanolic extract of Nymphaea Alba flower at the doses of 100mg/kg and 200mg/kg of body weight in mice.Diclofenac sodium used as standard drug Exhibited dose dependent and significant antiinflammatory activity in acute (acetic acid-induced vascular permeability, p< 0.001) and chronic (cotton pellet granuloma < 0.001). But at the dose of 200mg/kg of extract shown high significant than 100mg/kg. Hence, the present investigation established ethanolic extract of Nymphaea Alba flower has anti-inflammatory activity.