Background: According to National Institute of Occupational Safety and Health, computer vision syndrome affects 90% people who spend more than 3 hours a day on the computer. It is a group of eye and vision related problems. It is a temporary condition resulting from focusing the eyes on a computer display for prolonged, uninterrupted period of time. Also different reactions of the eye and the brain to the characters on the screen cause strain. Hence, this study is planned to assess computer vision syndrome in software professionals. Objectives: To assess Computer Vision Syndrome by history and clinical examination. Material and Methods: An assessment survey was conducted in a convenient software company. A total number of 60 people in age group 28-40 years having minimum exposure of three years to computer everyday were recruited in the study. A questionnaire was developed to collect data about perceived symptoms on computer vision syndrome. Results: It was observed that 80% of subjects suffer from backache, wrist and shoulder pain. 72% subjects complained of eyestrain and 70% complained of dry and irritated eyes. 62% subjects complained of headache. More than 50% subjects gave history of watering and redness of eyes. Conclusion: Study shows that more than 50% subjects suffer from some or the other symptom of computer vision syndrome. Early detection and prevention of computer vision syndrome is necessary to prevent future complications and better health of software professionals.