Objective: The current in vitro study was performed to evaluate the effect of fluoridated mouthwashes and immersion time on the amount of the metal ions released from titanium orthodontic mini-implants. Methods: Total 15 titanium mini-implants were used in the current study, which were divided equally into 3 groups (5 mini-implants in each group) and were immersed separately in (artificial saliva, Lacalut white and Kin B5 mouthwashes) for 28 days as following immersion intervals (1-7), (8-14) and (15-28) days. All mini-implants were assessed for metal ions release after immersion in the storage media. The mini-implants were used in as-received condition without any additional treatment. Results: The results of the Atomic Absorption Spectroscopy (AAS) showed that the release of total ions was highest in Lacalut White followed by Kin-B5 fluoridated MWs being higher than that released in artificial saliva, this was related to the pH of immersion media as well as the fluoride ion effect on the pattern of metal ions released from dental alloys. Conclusion: The factors of exposure time, alloy type and the type of the storage medium (pH and fluoride) influenced the pattern of metal ions released from MIs.