Objective: This study aims to assess the efficacy of first aid training provided to academic and administrative staff working at a state university. Materials and methods: The study sample included 318 academic and administrative staff members from a state university. Between the dates of December 15, 2017 to December 22, 2017, the study participants attended basic first aid training, the sessions of which lasted for 2 hours. Before and after the training, participants answered a 20-question survey questionnaire. Frequency, mean, Mann-Whitney U Test, Kruskal Wallis Variance Analysis, Wilcoxon T-test, McNemar chi-square analysis, and Spearman correlation for the analysis between variances, were used for the statistical assessment of data. Results: The mean age of the participants was 35.96 ± 8.96, 68.6% were female, 54.7% held a bachelor’s degree. The mean posttest score of the participants was found to be 75.0 ± 15.6, which was significantly higher than their mean pretest score of 60.0 ± 14.5 (p<0.05). Significant differences were found in terms of gender, level of education, and having undergone first aid training before and first aid knowledge scores (p<0.05), and a negative significant relationship was found between age and first aid knowledge scores. Conclusion: Results of the study showed that the first aid training provided to participants substantially increased their knowledge level, which strongly suggests that the training was effective. It is recommended that training can be repeated periodically, and they must be made known and available to all segments of society through the cooperation of various institutions and organizations.