The objective of this study was to determine the biochemical and hematological profiles of type 2 diabetic patients living with HIV. A total of 260 participants including 100 HIV positive and 160 HIV negative diabetics participated. Blood samples were obtained by venipuncture of the elbow crease. They were used to obtain plasma and serum. Serum was used for the determination of creatinine, total cholesterol, HDL cholesterol, LDL cholesterol, triglycerides, magnesium, phosphorus, urea, and cystatin C, while plasma was used to measure the level of glycated hemoglobin, blood sugar and perform blood count. The respective values of magnesium and phosphorus of DM/HIV+ (22.64 ± 5.72; 39.49 ± 9.66 mg/l) were significantly (p=0.000; <0.05) higher than those of DM/HIV- (20.11 ± 4.63; 35.81 ± 4.51 mg/l). The hematological parameters such as red blood cells, hemoglobin, hematocrit, lymphocytes, and basophils of DM/HIV+ were significantly lower than those of DM/HIV-. The GMV, MCHR, and platelets of HIV-positive patients were significantly higher than those of HIV patients.