In this study, a comparative analysis of angiogenic factor levels from Platelet Rich Fibrin (PRF) obtained from titanium and glass tubes was aimed. Angiotensin-Converting Enzyme (AGE), Interleukin-1a (IL-1a), Platelet-Derived Growth Factor (PDGF), and Interleukin-4 (IL-4) levels of PRF obtained from rats were determined by ELISA (Enzyme-Linked ImmunoSorbent Assay). According to the results obtained; AGE values in the Titanium-Platelet-Rich Fibrin (T-PRF) group were higher than in the control serum group. The mean IL-1a values in the glass tube (G-PRF) group were statistically higher than the other groups. IL-4 levels were found to be high in the G-PRF and T-PRF groups but were not significantly significant. PDGF values were higher in the T-PRF group than in other groups. This study, according to our literature research, is the first study showing the growth factor and cytokine levels in PRFB.