Pseudomonas aeruginosa is a cause of nosocomial infections that can be destroyer by antibiotic-resistant strains. This study conducted to determine the antibiotic susceptibility pattern and distribution of exoU and exoS among clinical isolates of P. aeruginosa. Fifty three specimens of tracheal tube were collected from patients who were hospitalized in ICU wards and P. aeruginosa were isolated and identified by phenotypic and molecular methods. Antibiotic resistance performs by disk diffusion and analyzed their virulence factors genes by PCR method. Susceptibility pattern of 53 isolates of P. aeruginosa showed that majority and minority of resistance belong to cefepime (55.4%),and Meropenem (50%) Respectively. Twenty four (45.2%) isolates were not susceptible to three or more different groups of antibiotics. Forty (71.4%) of isolated have had exoSand1(1.8%) exoU, 8(15%)both of exoS and exoU and the rest being negative for exoS or exoU. Distribution of MDR(resistance to three or more group of antibiotics) exoenzymes were shown: exoU(7.5%)and exoS(90.5%). According to statistically analysis there were not significant relationship between presence of exo SandexoU and antibiotic resistance.