Background and purpose of study: Amniotic fluid volume varies according to different stages of fetal growth and its different requirements. Disrupted amniotic fluid volume is associated with an increased risk for the fetus. The present research aims to investigate the effect of cutoff point amniotic fluid index on pregnancy prognosis at the third trimester of pregnancy in Shariati hospital of Bandar Abbas. Materials and methods: In the present analytical, cross-sectional research, AFI ≤ 5 cm was considered as oligohydramnios; AFI 5.1-8 was taken as the cut-off point; AFI ˃ 8.1-24 was regarded as normal; AFI ˃ 24 was considered as polyhydramnios. The data were analyzed via SPSS version 16.0 using Chi-squared test, Fisher’s test, Mann-Whitney U-test and Spearman’s correlation coefficient. P-value was set at ≤ .05 for the significance of data. Findings: Subjects with cut-off point AFI (5.1-8) were 38 (40.4%); those with normal AFI (8.1-24) were 56 (59.6%). The mean score of AFI was 8.85±9.54cm. The mean age of mother participants was 28.01±6.07 yrs. The mean length of pregnancy was 2.04±37 weeks. The mean gravity score was 1.46±2.4. The mean Apgar score 5 minutes after birth was 1.17±8.52. Quantitative variables were compared between the research groups but showed no statistically significant difference (p˃.05). Correlation of AFI and the target qualitative variables was assessed and the only significant correlation showed to be between gender and AFI (CI 95%=1.217-6.860, OR=2.88, P=.015). Conclusion: When AFI was at the cut-off point, fetal and infantile consequences were not significantly different from when AFI was normal. The risk level was low and AFI was higher among female than male feta.