Objective: The study aims to compare the cyclic fatigue resistance of One G, ProGlider, HyFlex EDM gpf, WaveOne Gold Glider in a single and double curved simulated canals. Method: Total 120 glide path files were included and divided into 4 groups (n=30), and were then subdivided into 2 group (n=15) and were used in single and doubled curved canals. The NCF (number of cycles to fracture) was recorded and measured the fragmented length. Data were analyzed using ANOVA, post-hoc Tukey. Result: NCF were lower in double curved canal compared to the single curved canal (p<0.05) in all the groups. Both single and double-curved canals, NCF of WOG Glider group was significantly higher than other groups (p<0.05). In the doubled curved canal, NCF of HEDM gpf was higher than PG group (p<0.05), while in single curved canal value of HEDM gpf group was non-significantly different than PG group (p>0.05). In both single and double-curved canals, NCF of the PG group was higher than the OG group. No statistical difference in fragment length among instruments was used in single and double curved artificial canals. Conclusion: WOG Glider had a higher cyclic fatigue resistance than another glide path in single and doubled curved artificial canals.