Abstract

Cytotoxic effect of Prangos Pabularia extract on HELA cell line a medicinal plant

Author(s):Sakinehsalehi, Saber Zahri and Seyed Mehdi Razavi

Cancer is regarded as a complex disease which is non-curable. Traditional medicines have been applied for the treatment of cancers in the world. Prangospabularia DC. (Apiaceae) is a perennial herb native to mountain slopes of the central and western Asia countries. Prangos species are widely used in folk medicine. Anticancer activity of Prangospabularia extract against HeLa line was studied using cytotoxicity effect. Many antitumor agents, have been reported to induce apoptotic cell death. This cell death plays a critical role in killing tumor cells in canser therapy(1) . In order to evaluate of cytotoxicity and antioxidant properties of the plant, dried powdered of roots of P.pabularia were Soxhlet extracted successively with n-hexan, dichloromethane (DCM) and methanol. Viability and cytotoxicity of HeLa cell line were measured by MTT assays. The antioxidant potential of the plant extracts was evaluated by DPPH assay. Additionally characteristic of cell death were using H&E morphological staining. The DCM extract showed a significant antioxidant effect with RC50 value of 0.08 mg/ml and our results showed that DCM extract displayed a significant cytotoxic activity against HeLa cell line in culture with a IC50 value of 0.526 mgml-1 in 24h with MTT assay. Additionally characteristic of aponecrotic cell death confirmed using H&E morphological staining. This study reveals biological effects and antitumor activity of Prangospabularia DC.(Apiaceae) extract against HeLa line.

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