Abstract

Detection of mecA Gene Associated with Methicillin Resistant Staphylococcus aureus and its Alternatives using Nanoparticles and Chia Seeds

Author(s):M. Rajamani, Jesteena Johney and R. Ragunathan

Staphylococcus aureus, a member of the family Micrococcaceae, is a Gram-positive coccus whose cells tend to occur either singly or if dividing cells do not separate, form pairs, tetrads and distinctive irregular “grape-like” structures, which is resistant to few antibiotics like Methicillin which is termed as Methicillin resistant Staphylococcus aureus. MRSA was isolated from the pus sample. Confirmation of MRSA was done by using Kirby Bauer disk method. Followed by sub culturing in Luria bertani broth and DNA isolation was performed by phenol chloroform method and confirmed by AGE (Agarose gel electrophoresis). The amplification of mecA gene thermocycler-PCR was done. Restriction fragmented linked polymorphism was done for knowing how much restriction sites are available for organism. To calculate the molecular weight of the protein SDS-PAGE (Sodium dodecyl sulphate - Polyacrylamide gel electrophoresis) was performed. Followed by alternative measures was done, by using Silver nanoparticles at different concentrations and Chia seeds against MRSA.

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