Purpose: Kinesio Taping (KT) is a technique that has been used in the clinical management of people with chronic back pain. This study investigated the efficacy of KT on patient with chronic non-specific low back pain using electromyography (EMG) and three-dimensional motion analysis (3DMA). Subjects: 50 patients with chronic low back pain aging from 25 – 40 years, with mean age (36.62±2.9) years. Patients were divided randomly into two equal groups, placebo group (A) received sham KT, and group B received real KT. Methods: The outcome measurements were electrical activity of lumbar Para spinal muscle using EMG pre and post KT, lumbar curvature using 3DMA and pain Pre and post KT using visual analogue scale (VAS) EMG and 3DMA were carried out at baseline and 2 weeks later while pain was recorded after 1 month. Results: Paired analysis for comparison between pre and post treatment measurements in each group showed significant decrease of lumbar curvature as well as medium frequency of Para spinal muscles in group B than group A. also there is significant decrease of pain in group B than on group A. Despite the equal baseline of all groups before treatment, there were significant decrease of lumbar curvature, medium frequency of Para spinal muscles and pain measurements in real KT group than placebo group. The results suggested that kinsiotaping have beneficial effects on pain, range of motion, and trunk muscle endurance in people with chronic non-specific low back pain of mechanical etiology.