Background/Purpose: The demand for invisibility appearance of fixed orthodontic appliances result in increase the use of ceramic brackets with clear auxiliaries. Elastic ligatures are one of the most widely used materials in fixed orthodontic treatment and susceptible for discoloration. The aims of this study were the evaluation and comparison the effect of brushing orthodontic clear elastic ligatures by whitening toothpastes with different whitening agents on the color stability of them. Materials and method: The sample consisted of whitening toothpastes: Kin Progressive White (Fabrique par, Barcelona, Spain), Lacalut White (Germany), Silca Brilliant White (Germany), and Opalescence White (UT, USA), also Kin Regular (Fabrique par, Barcelona, Spain) (control). Sixty Ormco Clear Ligatures (Scafati, Italy) used in the study, these ligatures exposed 1 hour daily to dietary media (Mixture of tea, coffee, turmeric, and mineralized water) (pH=4.6) and each 10 specimens brushed with one type of toothpaste, except 10 elastics without brushing. After 4 weeks, the ligatures were assessed by a cellular attachable microscope that connected to the mobile phone according to CIE L*a*b* color system by the Adobe Photoshop program. Results: The resulting data were statistically analyzed using ANOVA and Tukey’s HSD tests which showed that the highest mean value of color change was observed in no brushing ligatures followed by Silca Brilliant White, Lacalut White, Kin Progressive White, Opalescence White, the lowest value of color change was Kin Regular. Furthermore, the increased mean value of color change was mainly affected by (CIE b* axis). Conclusion: Kin Regular toothpaste without whitening agents decreased the color change of clear ligatures more than others after exposure to staining media.