People with certain blood groups and Rh positive are more prone to infections transmitted by blood transfusion. The aim of this research was to survey the of accompaniment of ABO Blood Group System and Rh type with infection to hepatitis B virus and hepatitis C virus in Hamadan Blood Transfusion Organization, Iran. This was a retrospective study in patients during years 2006-2013 in Hamadan blood transfusion Organization. The population of blood donors were tested for blood borne infections, including HBsAg and HCV. Logistic regression was used and collected data were analyzed using SPSS version 16.The number of 1036 cases of referred population had a background of infected hepatitis B and C. Different blood groups had not a significant effect on the incidence of hepatitis B and C viruses. But the Rh difference had a significant effect so that people with Rh-negative, had the risk of incidences to hepatitis B as 0.53 folds than positives while people with Rh-positive, had the risk of incidences to hepatitis C as 1.87 folds than Rh-negatives. This study emphasizes that there is a more possibility for transmission of HBV and HCV infections for people with negative and positive Rh more than others do but it cannot be referred to a specific blood group. It is recommended that in planning of disease prevention in the country, broad spectrum of research will perform about the role of ABO system and Rh in afflicting to viral infections as the priority for sanitation authorities.