Barbershops are considered as public places and can contribute to the transmission of many diseases. In this study, samples were obtained from a total of 18 barbershops in Kamyaran, Iran, to evaluate the type of bacterial and fungal contaminations. Sampling was carried out through sterile swab in both blood agar and Saborad Dextrose agar mediums. After identification of the bacteria through biochemical tests and determining their count, the relationship between type and amount of contaminations and several other factors were examined through statistical tests. a total of 6 bacterial species including gr- Bacilli, gr+ cocci, Micrrococcus, Staphylococcus, and gr+ Bacilli and 7 fungi species including Cladosporium, Trichophyton, Mucor, Aspergilus, Candida, Penicilium, Rhizopus were isolated and identified. Staphylococcus with a frequency of 29.6% and Aspergilus fungi with a frequency of 26.5% were the most frequent bacterial and fungal colonies, respectively.