Aim: To evaluate the cyclic fatigue resistance for the new reciprocating glide path file Wave one gold glider, Proglider, Pathfile and path glider komet. Materials and Methods: Forty instruments were used in this study divided into four groups (n=10): G1: Path file, G2: Path glider komet, G3: Wave one gold glider, G4: Proglider. The instruments were tested for their cyclic fatigue resistance by an artificial canal 60° angle of curvature with a width of the canal was 1.5 mm and radius of curvature for both canals was 5 mm within a block, cyclic fatigue resistance data were analyzed with one way ANOVA and Tukey test. Result: There was a high significant difference (P<0.000) between tested instruments in ANOVA and Tukey test, with the highest cyclic fatigue resistance value produced by Wave one gold glider. Conclusion: Wave one gold glider reciprocating path glider instruments had the highest cyclic fatigue resistance with high flexibility.