Background: In the present study, we investigated the role of cadmium in acute liver injury, as well as the underlying mechanisms. Methods: On the other hand, we investigated whether Punica granatum flower extract (PG) has a healing effect on CD-induced liver damage. Control groups (G1-G2) received water and 200 mg/kg PG, respectively. Toxicity groups (G3-G6) received 2 different doses of Cd (15-30 mg/kg b.w) orally in a single administration. To evaluate liver function, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and γ-glutamyl transferase (GGT) were detected in the serum, superoxide dismutase (SOD), glutathione peroxidase (GPx), thiobarbituric acid reactive substances (TBARS) were established in the liver and 8-hydroxy-20-deoxyguanosine (8-OHdG) levels were determined for the antioxidative and anti inflammatory effects of PG. Tissue sections were also evaluated histopathologically. Results: We found that Cd exposure decreased levels of SOD, GPx and, increased expression of caspase-3 and levels of TBARS and 8-OHdG. Biochemical and histopathological analysis revealed the toxic effects of cadmium on the liver for the rats in oral acute toxicity study. Functional parameters were significantly improved in PG-treated groups and the severity of the liver injury and apoptosis were significantly decreased in this group. Conclusion: As a result, PG can be consumed as a protective agent against acute liver injuries.