In the absence of confirmed causes for around 50% of recurrent pregnancy loss (RPL) cases this study was conducted in order to evaluate the relationship between PAEP gene single nucleotide polymorphism (SNP) rs760140467 C>T and recurrent pregnancy loss (RPL) in Palestinian women. A retrospective case-control study was carried out during the period (August 2015 to April 2016). A total of 200 females, 100 RPL patients and 100 control women without previous history of RPL were included in the study. PAEP (rs760140467 C>T) polymorphism was tested by PCR-RFLP. Statistically significant difference existed between RPL cases and controls in terms of the genotypic and allelic distribution of the tested polymorphism. PAEP "T/C" genotype and "T" allele were significantly higher in the RPL group. The study showed, for the first time, that the "C/T" genotype and the "T' allele of the tested polymorphism are strongly associated with RPL in the investigated population. This finding may lead to improved therapeutic approaches for those RPL cases.