Background: Nursing is one of the professions which require constantly being involved with people. Based on the negative work conditions, job satisfaction of the nurses may decrease and their burnout levels may increase in the long term. Objective: This study was conducted with the aim of identifying the burnout and job satisfaction levels of the beginner nurses. Methods: The sample of the research comprised nurses who newly started working at a university hospital in Istanbul (n=218). Nurse information form, Maslach Burnout Inventory, and the Minnesota Satisfaction Questionnaire were used for the research. Results: It was identified that 91.28% of the nurses were women, burnout level of the nurses was low and job satisfaction level was high. A statistically significant positive relationship was present between the burnout and job satisfaction levels (p<0.05). Conclusions: It was detected that the beginner nurses who were satisfied with the unit they were working at had a low level of emotional burnout and high level of job satisfaction. It was also identified that nurses who willingly chose the unit of work to have high levels of job satisfaction. Providing well-planned orientation training could be helpful for the beginner nurses to adjust and to embrace the work environment.