International Journal of Medical Research & Health Sciences (IJMRHS)
ISSN: 2319-5886 Indexed in: ESCI (Thomson Reuters)

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In vitro Anti-Candidal effects of Aqueous and Methanolic Extracts of Walnut (Juglansregia) Tree Fruit Peel in Comparision with Fluconazole

Author(s):Ali Naseri, AbdolmajidFata and Seyyed Ali Akbar Shamsian

The appearance of fungal species resistant to antifungal drugs among the Candida genus and also their numerous side effects, convince the researchers to work on new therapeutic methods with minimal side effects for humans fungal infections. The aim of this study was to assess of anticandidal activities of aqueous and methanolic extracts of walnut Fruit Peel on common Candida species. In this study, the antifungal effects of aqueous and methanolic extracts of fruit peel of walnut (Juglansregia) and fluconazole against four Candida species were evaluated using broth Microdilution method, based on the Clinical and Laboratory Standards Institute (CSLI) M38-A3 guideline. And then the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) of these extracts and antifungal drug were obtained for tested Candida species. Both aqueous and methanolic extracts of fruit peel of walnut showed antifungal effects against Candida species. The MIC of fluconazole, aqueous and methanolic extracts of walnut fruit peel for different Candida species were 0.001- 0.032, 6.25-50, 3.125-25 mg/ml, respectively. The MFC of fluconazole, and methanolic extracts of fruit peel of walnut were 0.001-0.032 and 6.25-25 mg/ml, respectively. The aqueous extracts of walnut fruit peel were without fungicidal effects. The most important result of this study is to show that fruit peel extracts of walnut are effective against named fungal species and it looks promising that in future, we can obtain some effective antifungal agents with minimal side effects from shallot extract.

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