Introduction: Stress, defined as an imbalance between environmental conditions necessary for survival and the ability of individuals to adapt to those conditions, have a high prevalence in MBBS students. A variety of stressors play a significant role in developing stress. Objective:To study the level of stress and stressors responsible in Final MBBS students of Rural Medical College, Loni. Methods: A descriptive cross sectional study was carried out in 100 students (50 of either sex) willing to participate in the study. They were subjected to fill the Medical Student Stressor Questionnaire, which consists of 40 questions for evaluating the stressors and severity of stress perceived by the subjects. Results: 71% subjects’ perceived moderate stress, while 13% and 16% perceived high and mild stress respectively. Academic stressor counted for moderate stress in 63% and high stress in 24 % of subjects, which was higher than other stressors. Conclusion: Academic stressors being the major stressor perceived, Strategies are required to decrease the burden of academic stress in the students.