Background: Low back pain influences majority of the population, especially in working-age in modernity. It interferes with activity of daily living. A low frequency magnetic field therapy field has been advantageous in patients suffering from low back pain. Methods: The present study evaluated the effect of low frequency magnetic field therapy (1 Hz and 100 Hz) with intensity up to 100 Gauss per particular output on visual analogue scale for pain scores and baseline bubble inclinometer for forward and lateral (right and left) trunk flexion mobility in forty patients (22 males and 18 females) suffering from low back pain, their age ranged from 40-50 years old who received either low frequency magnetic field therapy as experimental treatment or sham exposure treatment. Results: Repeated analysis of study outcome measures pre-treatment, post-treatment, and follow up periods showed a significant difference in visual analogue scale (pain) and trunk mobility, (p<0.05). On the other side, there was no significant difference in sham group during evaluation periods in both outcome measures (p>0.05). Conclusion: The findings from our study give initial support for using low frequency magnetic field therapy as a long term effective treatment method for decreasing pain and increasing trunk mobility.