Background: Male infertility due to severe oligozoospermia and azoospermia has been associated with a number of genetic factors. Chromosomal abnormalities are one of the principle genetic factors in male infertility. Purpose: To detect the frequency and types of chromosomal anomalies in azoospermics and severe oligozoospermics in Western region of India and to compare these with same studies of other regions of India and the world. Method: - To assess the chromosomal aberration, the karyotyping of 44 azoospermics and 68 severe oligozoospermics were performed on peripheral blood lymphocytes using standard G-banding technique. Result: Chromosomal anomalies in azoospermics 18.2% (n=8/44) and in severe oligozoospermics 7.35 % (n=5/68) with an over all 11.6% (13/112) in western parts of India were comparable with studies from Europe, Africa, Asia, South America and other regions of India. Polymorphic chromosomal variants were high 36.6% in infertile males; this incidence was similar 33.3% that found in controls. Conclusion: In view of the genetic risk for the next generation, the occurrence of chromosomal anomalies among infertile males strongly suggests the need for routine genetic testing and counseling prior to employment of assisted reproduction technique. It should be discussed during genetic counseling.