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International Journal of Medical Research & Health Sciences (IJMRHS)
ISSN: 2319-5886 Indexed in: ESCI (Thomson Reuters)

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Abstract

Mechanisms Underlying the Relaxation Induced by Bradykinin in Rat Aorta

Author(s):Chinar M. Mohammed and Omar A.M. Al-Habib

Bradykinin (BK) plays a critical role in intervening relaxation in vascular smooth muscle through distinctive mechanisms. The current project is designed to study the role of PKA, calcium-dependent potassium (BKca, IKca,andSKca) channels, voltage-dependent potassium channel (Kv) and inward rectifier potassium channel (Kir) in BK interceded response and to determine functionally the mechanisms underlying BK mediated relaxation, utilizing standard tissue bath protocols. The results of the current work showed that BK inducing vasorelaxation is mediated by PKA which activate eNOS (endothelial nitric oxide synthase) followed by a subsequent release of nitric oxide (NO) from endothelial cells since the protein kinase inhibitor (H-89) significantly reduced Emax and pIC50. Similarly, in rings pre-incubated with L-NAME (3×10-4M) significantly inhibited vasorelaxation induced by BK. Furthermore, both Kca and Kv played an interesting role in vasorelaxation mediated by BK (1×10-6 to 3×10-3 M) in phenylephrine (PE) precontracted aortic rings, pre-incubated with tetraethylammonium (TEA) which reduced Emax with a slight reduction of pIC50 as compared to the control. Also, aortic rings pre-incubated with IK(Ca) blocker (clotrimazole) markedly reduced Emax but with a tendency of pIC50 elevation. In the present study, voltage-dependent potassium channels blocker (4-AP) significantly reduced the relaxant effects of BK and reduced Emax with altering pIC50 values significantly. On the other hand, BaCl2 tends to enhance the relaxation induced by BK and significantly elevated Emax. Collectively, our findings indicate that BK-induced relaxation is dependent on the activation of PKA to release NO from endothelium which through a cascade of a signaling pathway, in turn, activate both IK(Ca) and Kv in rat artic vascular tissue.


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