Nosocomial infections or healthcare-associated infections happen in patients under medical care, which constitute nearly 50% of all hospital infections. Human Immunodeficiency Virus (HIV) infected patients are more prone to get infected in hospitals. In the present study, the frequency of different bacteria causing Nosocomial Urinary Tract Infection (NUTI) among HIV positive and HIV negative groups were investigated. 80 immunocompetent and 80 immunocompromised (HIV/AIDS) admitted patients, who had developed signs and symptoms of UTI after 48 h onwards from the day of admission, were selected for the study. There was significant variation (p<0.001) between the presenting symptoms of the two groups, especially, dysuria. Escherichia coli were the predominant microorganism found in the urine of both groups. Other than that, Acinetobacter spp., Morganella morganii, Serratia marcescens, and Staphylococcus epidermidis were only noted in HIV-positive patients. Serratia marcescens has been newly identified in the host institution as a urinary tract pathogen. Perhaps, ampicillin, amoxicillin, clavulanic acid, cephalosporins, nitrofurantoin, co-trimoxazole were significantly resistant among gram-negative isolates. These findings could be attributed to the fact that the immune systems were more vulnerable in HIV-positive groups, making them susceptible to organisms that are not generally considered pathogenicy.