Monitoring of negative effects of the chemical additives in food and environment is an important function of quality control laboratories around the world. Therefore, the aim of this study is to evaluate the effect of various doses of azorubine (AZ) on ovary histopathology in female Sprague Dawley® rats, in addition to testing some of the hematological parameters and hormones of the experimental groups. We found that there were various moderate to severe changes in the ovary histopathology at different doses of treatment. As well as, the hormone levels are affected drastically by the effect of different doses of AZ. On the other hand, hematological outcomes are not so significantly affected by this synthetic dye. Finally, we conclude that AZ can be considered as one of the most important causes of infertility, hormonal disturbances and irregular menstruation in the female that should be banned from the foodstuff that are imported to our country from other countries.