Context: The prevalence of occupational health hazards and mortality has been reported to be unusually high among people of India. Although developed countries are very much careful about the health in occupations it is quite neglected in the developing countries like India. Aims: To record PEFR in asymptomatic male workers exposed to chemical fumes for more than 2 years and compare the results with age matched unexposed, healthy male controls. Methods and Material: This was a comparative study between 50 asymptomatic male workers exposed to chemical fumes for more than 2 years in various industries located at Jeedimetla Industrial Area and 50 unexposed healthy male individuals from general population. The sampling was done by simple random sampling (lottery method). The data was collected in the Research Laboratory of Physiology. Anthropometry like weight, height, was measured and the PEFR test was performed in the standing position by taking a deep inspiration and then blowing out as hard and as quickly as possible with their nose closed. Data was analyzed by using SPSS package and was expressed in terms of mean ± SD. Results: It was observed that mean PEFR was statistically highly significant in cases (p = 0.0001), and PEFR decreased with increase in duration of exposure. Conclusions: Thus, it can be concluded that apparently healthy individuals may also have abnormal PEFR findings. Hence, a regular check on these parameters will help them in reducing the chances of its manifestation at a future date.