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International Journal of Medical Research & Health Sciences (IJMRHS)
ISSN: 2319-5886 Indexed in: ESCI (Thomson Reuters)

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Abstract

Polymerase Chain Reaction Can Run Faster by Parameter Optimization

Author(s):Ruyue Luo, Sijie Gan and Yongping MA

For a conventional PCR, the total reaction times of a certain DNA amplification using conventional PCR were minimized to more than a half. The extension time could be calculated as the formula: y=0.005x-5.2679 (y represented the extension time (sec) and x represented the DNA length (bp); y ≥ 1.0). The annealing time was minimized as short as 1 sec. However, the Ta was changed from Tm ± 6.0°C.


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