Objectives: In March 2020, the World Health Organization declared Coronavirus Disease 2019 (COVID-19) as a pandemic. This study aims to measure the prevalence of COVID-19 and epidemiological features of laboratoryconfirmed COVID-19 cases in the Jeddah Region, Kingdom of Saudi Arabia (KSA). Methods: A multi-center, analytical, cross-sectional study was conducted on 21,272 laboratory-confirmed COVID-19 cases from March 1 to May 31, 2020, in Jeddah Region, KSA. The cases included all ages and genders with no exclusion criteria. We estimated a confidence interval of 95%, a margin of error of 5%, and a response distribution of 50%. Using this information, we calculated the sample size using Raosoft as 416 cases. Data variables included demographic data, and information such as travel history, status as healthcare workers, and others variables. Results: The prevalence of COVID-19 in the Jeddah Region was 4.2 per 1,000 people (0.42%). The average age was 37.80 ± 13.5 years. The majority of participants were male (76.9%). Recovery outcome classifications were observed in 95.7%. Most of the participants were non-healthcare workers (85.4%). Travel information showed that most participants had no history of travel inside Saudi Arabia (88.8%). Conclusion: In this multi-center cross-sectional study, the age ranged from 18- 35 years, and those who are the case with no contacts are the strongest risk factor for COVID-19 outcomes. Therefore, implementing well-design strategies to break the chain of infection is necessitated. Wearing masks, social distance, and immediate patient isolation play a significant role in controlling the transmission of COVID-19.