UTI, the most common bacterial infection in urinary tract, is a serious health- problem that occurs in millions of people at any age in each year. Its empirical treatment is difficult worldwide. Local susceptibility-pattern of uropathogens is, therefore, important. To determine prevalence of UTI-associated uropathogens and their antibacterial sensitivity-pattern. The midstream urine samples were collected from patients of different age-groups, followed by examination with semi-quantitative culture method and determination of antibacterial sensitivitypatterns using Kirby-Bauer disc diffusion technique. Data were analyzed in MS-Excel, 2007, and chi-square was used to test the significance. Out of 4872 samples, 34 % showed significant growth of pathogens. The patients were from newborn to 80-years-old. Majority of UTI (55.2 %) were from middle-aged patients with 20-49 years-old and 83.9 % organisms were isolated from females. The gram-negative aerobic rods accounted for 83.11 % prevalence and that of gram-positive was 16.88 %. The infections caused by E. coli (44.33 %), Klebsiella spp. (28.23 %), S. saprophyticus (8.32 %), and S. aureus (6.27 %) were prevalent in middle-aged females. These females were markedly associated with pathogens (χ 2=25.14, p<0.001). The drugs such as levofloxacin, ofloxacin, ciprofloxacin, ceftriaxone and cefotaxime were five most sensitive antibiotics. Conclusion: Out of 4872 samples, 34 % showed significant growth. Females were markedly associated with pathogens (χ 2=25.14, p<0.001). The infections caused by E. coli, Klebsiella spp., S. saprophyticus and S. aureus were predominant and drugs like levofloxacin, ofloxacin, ciprofloxacin, ceftriaxone and cefotaxime the sensitive antibiotics.