Intestinal parasites constitute major health problems, especially in the tropical and subtropical regions. In developing countries, it is estimated that 3.5billion people are affected and 450 million are ill as a result of these infections, the majority of them being children. This was a cross-sectional school based study. It was conducted in the primary schools of Alhag yousif, East in East Nile locality, Khartoum State and aimed to estimate the distribution of intestinal parasites among the school children in the study area. 120 stool samples were collected and analysed by direct saline stool preparation and Formol-Ether Concentration Technique. Results shows that, (84%) stool samples were positive by the Formol-Ether Concentration technique. While (65%) were positive by the direct saline stool preparation. Intestinal parasites were more prevalent among the male students (80%) than the females (60%). Furthermore, they were more prevalent among the age group 5 to 7 years old (5%). The study concludes that intestinal parasites were more prevalent among the male students and among the age group 5to 7 years old. The study recommends using Formol-Ether Concentration technique for diagnosis of intestinal parasites.