Aim of the study: To test the pervasiveness of vaginal infection in diabetic pregnant women and normal pregnant women; Al Dawadmi, Saudi Arabia. Patients and methods: The samples are obtained from Al- Dawadmi hospital, Riyad, Saudi Arabia. If vaginal swab samples positive microbial tests for any infection and culture in media, we were reported the participants and referral to the gynecologist. Vaginal swabs were gathered from 500 pregnant women divided into 250 diabetic and 250 non-diabetics. The ages were ranged from 20 to 45 years. Results: Total 500 women were included in this study. Of these patients, 253 (50.6%) of them diagnosed vaginal infection. 30.6% was diagnosed with diabetes and 20% was diagnosed with non-diabetes. This prevalence is comparably higher among pregnant women with diabetes. Conclusion: The ordinary microbiota may don’t allow infection and colonization of the host and the spread of microorganisms related to urogenital infections, including those in charge of bacterial vaginosis, fungal, viral, protozoal and aerobic bacterial vaginitis, the disturbed vaginal flora, particularly when asymptomatic, could cause female and male problems, infertility and be principally connected with an adverse pregnancy outcome, as well as maternal and fetal morbidity.