Mouse bone marrow mesenchymal stem cells (mBMSCs) soup is promising tool for the treatment of neurodegenerative diseases. mBMSCs soup is easily obtained and is capable of transplantation without rejection. We investigated the effects of mBMSC soup on staurosporine-induced cell death in PC12 and U87 cells lines. The percentage of cell viability, cell death, NO concentration, total neurite length (TNL) and fraction of cell differentiation (f%) were assessed. Viability assay showed that mBM soup (24 and 48h) in time dependent were increased cell viability (p<0.05) and also cell death assay showed that cell death in time dependent were decreased, respectively (p<0.05). TNL and fraction of cell differentiation significantly were increased compared with treatment1 (p<0.05). Our data showed that mBM Soup protects cells, increases cell viability, suppresses cell death and improvement the neurite elongation. We concluded that Mouse bone marrow mesenchymal stem cell soup plays an important protective role in staurosporine-induced cell death in PC12 and U87 cell lines.