GET THE APP

REAL TIME POLYMERASE CHAIN REACTION (RT-PCR) FOR MYCOBACTERIUM TUBERCULOSIS IN SERPIGINOUS CHOROIDITIS- A STUDY OF 29 CASES | Abstract
Logo

International Journal of Medical Research & Health Sciences (IJMRHS)
ISSN: 2319-5886 Indexed in: ESCI (Thomson Reuters)

Abstract

REAL TIME POLYMERASE CHAIN REACTION (RT-PCR) FOR MYCOBACTERIUM TUBERCULOSIS IN SERPIGINOUS CHOROIDITIS- A STUDY OF 29 CASES

Author(s):*Radha Annamalai, Jyotirmay Biswas, S Sudharshan , R Gayathri ,K Lily Therese, Viswanathan S, Namitha Bhuvaneswari

A study of real time Polymerase Chain Reaction for Mycobacterium tuberculosis (M. tuberculosis) DNA in 29 cases of active serpiginous choroiditis. Design: Case control study. Methods: DNA extraction from the aqueous humor was carried out using QIAMP DNA extraction kit. Real- time Polymerase Chain reaction (RTPCR) for MTB was carried out using Genosen’s Mtb complex quantitative Real time PCR kit. All patients were also subjected to complete blood count, venereal disease research laboratory test, chest radiograph, QuantiFERON TB Gold test on the blood and polymerase chain reaction on a sample of aqueous humor. Results: Aqueous aspirate showed copies of mycobacterium tuberculosis DNA in one out of twenty nine cases of serpiginous choroiditis. Direct smear and culture for mycobacteria was negative in all cases. Conclusion: RTPCR identifies MTB DNA in suspected latent tuberculosis in serpiginous choroiditis with high specificity. Serpiginous choroiditis and multifocal choroiditis due to tuberculosis may resemble each other clinically but have distinct clinical features which can be confirmed by real time polymerase chain reaction performed on the aqueous humor The association between serpiginous choroiditis and tuberculosis would be a chance association or if present a rare association.


Archive
Scope Categories
  • Clinical Research
  • Epidemiology
  • Oncology
  • Biomedicine
  • Dentistry
  • Medical Education
  • Physiotherapy
  • Pulmonology
  • Nephrology
  • Gynaecology
  • Dermatology
  • Dermatoepidemiology
  • Otorhinolaryngology
  • Ophthalmology
  • Sexology
  • Osteology
  • Kinesiology
  • Neuroscience
  • Haematology
  • Psychology
  • Paediatrics
  • Angiology/Vascular Medicine
  • Critical care Medicine
  • Cardiology
  • Endocrinology
  • Gastroenterology
  • Infectious Diseases and Vaccinology
  • Hepatology
  • Geriatric Medicine
  • Bariatrics
  • Pharmacy and Nursing
  • Pharmacognosy and Phytochemistry
  • Radiobiology
  • Pharmacology
  • Toxicology
  • Clinical immunology
  • Clinical and Hospital Pharmacy
  • Cell Biology
  • Genomics and Proteomics
  • Pharmacogenomics
  • Bioinformatics and Biotechnology
  • Google scholar citation report
    Citations : 2979

    International Journal of Medical Research & Health Sciences received 2979 citations as per google scholar report

    International Journal of Medical Research & Health Sciences peer review process verified at publons

    paper.io

    agar io

    wowcappadocia.com
    cappadocia-hotels.com
    balloon-rides.net

    wormax io

    Elektronik Sigara
    Elektronik Sigara