Proper leadership style helps proper functioning of nurses who are the most frequent experts in hospitals and improves quality of their work. One of the important factors affecting nurses` performance is the behaviors of managers toward them which is due to their personality type. The purpose of this study was to study the relationship between personality type and leadership style of nursing managers in Iran-Esfahan medical university hospitals in 2015. Present study is descriptive correlation type. The research community are 150 nursing managers of three levels, Matron, Supervisor and head nurse, from hospitals belonging to Esfahan medical university. Data collection was done by a 3-part questionnaire consisting of demographic information, McCurry and Costa 5-factor personality questionnaire and Renesis Likert questionnaire for determining leadership style. Data were analyzed using SPSS software ver. 22 in two level, descriptive and inferential. The average scores of dominant responsible personality among the first, second and third level were 36.33±5.12, 39.59±5.51 and 36.50±6.75, respectively and average scores of dominant organization oriented leadership style were 12.56±1.88, 11.03±4.54 and 12.30±4.40. Hospitals like other organizations require modern leadership styles instead of conventional one. Although organizationoriented leadership has been beneficial to achieve organizational objectives in short term, but in the long term it decreases productivity and job satisfaction because of lack of proper attention to the staff. Thus, applying humanoriented leadership, specially combined leadership will have much better results. Responsible personality is useful for the organization and increases productivity but it seems that in health care department in which interaction with humans is crucial, flexible or extroverted personality is more beneficial.