Herbs were always important in supplying hygiene and society health regarding the treatment and diseases prevention and it still does. Traditional medicine has been used since a thousand years ago for the therapeutic objectives and among them using essences and herbal plants has a considerable position. Our study showed thymol and carvacrol are main effective combinations of thyme and can influence warm positive and negative bacteria. Zataria multiflora Boiss. is a perennial plant which mainly grows in Iran, Pakistan, and Afghanistan. Zataria multiflora Boiss. is used in Iranian traditional medicine for its various effects including antiseptic, analgesic, and carminative properties. New researches showed it has analgesic, antispasmodic, antioxidant, antibacterial, antitussive, and anti-inflammatory impacts.