Introduction Human immunodeficiency virus (HIV) shares routes of transmission with Hepatitis B virus (HBV), so HIV patients have more chance to get co-infected with HBV and this type of concurrent infection with both viruses may alter the disease progression, natural history and treatment response. Material & Method The study was carried out at the Integrated Counselling and Testing Centre (ICTC) of Department of Microbiology, MBS Hospital, Government Medical College, Kota. The present study included 100 patients, diagnosed as HIV positive. Results Among the 100 HIV positive patients we found 35 patients co-infected with HBV. Among the 100 cases of HIV, 65 (65%) were male, 34 (34%) were female and 1 (1%) was intersexual. In HIV +HBV co-infected cases 22 (62.8%) were male and 13 (37.1%) were female. Of the 100 HIV patients most were married 73 (73%) followed by unmarried 16 (16%), widow 7 (7%), separate 4 (4%). Among HIV+HBV co-infection most was married 28 (80%) as compared to separate 3 (8.5%), unmarried, 2 (5.7%) and widow 2 (5.7%). Among the HIV patients route of transmission was mainly sexual 69 (69%).