Background &Aim:Numerous infectious diseases are spread by blood transfusion, particularly viral infections. The hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus (HIV) and other pathogenic organisms are transmitted through inappropriate screening of blood product. These infected blood products are causing fatal, persistent and life frightening disorders. The predominance of these viruses differs by ethnic group and geography. Scheme of the current study was to statistical estimation of the incidence of HBV, HCV and HIV along with blood donors. Materials & Methods: The existing review was approved in Ibn Sina Teaching Hospital, Sirt Region of Libya. A total of 16,929donors were analyzed by enzyme immune assay (EIA) kits from TaytecInc, Canada, for the predominance of human immunodeficiency virus, hepatitis B and C virus, over a period of 17 months from January2012 to May 2013.Results: Among the blood donors, 81.40% were unpaid donors and 18.60% were alternative donors. The total incidence in blood donors was 3.18%. The seroprevalence of hepatitis B was uppermost (1.98%) followed by hepatitis C (1.20%) and seroprevalence of HIV was nil among unpaid and surrogate donors. Conclusion: Present study was emphasized the prevalence rates of HBV and HCV between charitable and alternative blood donors and the HIVwas not detected in the current study.The prevalence rate was more in male among the blood donors.