The imidazole derivatives have potent therapeutic activity against cytotoxicity and parasites. The present study was planned to synthesize novel tetra aryl imidazoles compounds and evaluated for in vitro cytotoxicity and anthelmintic activity. Firstly, 2-amine-4-chloro pyridine was condensed with substituted benzaldehyde to give corresponding Schiff’s base. These Schiff’s bases further on treatment with ammonium acetic acid derivation and isatin yielded comparing novel tetra aryl imidazoles. The synthesized compounds were examined for in-vitro cytotoxicity and anthelmintic activity. The discoveries showed that all the synthesized novel substituted imidazoles have moderate to great anthelmintic action. They additionally had critical in-vitro cytotoxicity against HEp2 cell lines (Human larynx malignancy cell line) against standard utilizing 5-fluorouracil. The compounds 1b, 2b, 4b, 6b, and 8b had higher anthelmintic action contrasted with standard mebendazole. The synthesized compounds 1b, 2b and 8b had noteworthy in-vitro cytotoxicity against HEp2 cell lines.