This study investigates the bacterial colonies’ numbers in COPD patients. Two groups of people were included in this study; 25 healthy individuals’ groups and 32 COPD patients’ groups, which were further subdivided into three subgroups; 12 smokers, 13 secondhand smokers, and 7 nonsmokers. The sputum sample of each individual was collected to identify bacterial colonization. The results showed different types of bacterial isolates in the two studied groups. Streptococcus pneumonia and Staphylococcus aureus are the most common bacterial isolates in the patients’ group. The numbers of bacterial colonization among the COPD individual are greater than the healthy individual group.