Using educational methods among nursing students is very important, as they increase knowledge and affect their attitude and ultimately their performance. The use of an effective training method leads to reform and change in bad habits and replacement of correct hygiene habits. This issue is also very important element for providing, maintaining and improving the health of patients.The general purpose of this study was to compare the effect of two educational methods of film displaying and lecture on knowledge, attitude and performance of hand hygiene in nursing-midwifery students of Tabriz University of Medical Sciences. In this study, the research population included 5th and 6th semester nursing students of Tabriz University of Medical Sciences, School of Nursing and Midwifery, which are randomly selected based on inclusion criteria.This research is a clinical trial study. The data collection tool consists of 4 parts. In both group, demographic questionnaire, and questionnaire of knowledge, attitude and performance of hand hygiene before and after intervention were conducted. Collected data were analyzed using SPSS 17 software and they were analyzed using descriptive indices of mean, standard deviation and inferential statistics (paired t-test) and (independent t). The results of this study showed that there is difference between knowledge and belief of nursing students before and after training using film displaying method (p <0.05), while that is no significant difference between the performance of nursing students before and after training by displaying method. In addition, there is no significant difference between knowledge, belief, and the performance of nursing students before and after the training by lecture method.