Physical activity and diet are the most important modifiable determinants of cancer risk. The objective of this study was to examine the effect of intense intermittent training with and without taking vitamin E on expression of p53 and PTEN tumor suppressing genes in the prostate gland of male rats. For this purpose, 50 Sprague-Dawley male rats were randomly assigned into 5 groups:  control (CON, n = 10),  sham (S, n = 10),  intense intermittent training (IIT, n = 10),  intense intermittent training + vitamin E (IIT + VE, n = 10),  vitamin E (VE, n = 10). Protocol of this study was implemented for 6 days per week for 6 weeks, with observing the overload principle on the motorized treadmill. After implementing training protocol, expression rate of p53 and PTEN genes reduced significantly (p<0.000, p<0.031, respectively). Taking vitamin E with intermittent training caused significant reduction in p53 expression (p<0.013), while it caused significant increase in expression of PTEN (p<0.035). These results showed that intense intermittent training reduces expression of p53 and PTEN tumor suppressing genes and taking supplementation vitamin E along with this type of training could cause different effects in expression of these tumor suppressor genes.