Abstract

The Effect of Short-term Intraperitoneal Injection of Fe2O4Zn Nanoparticle on Liver Enzymes and Tissue in Male Wistar Laboratory Rats

Author(s):Somayeh Saadi, Zahra Hooshmandi, GhodousFathi and Farzaneh Janmohammadi

The present study sought to examine the short-term effect of Fe2O4Zn nanoparticle on histological and enzymatic changes of liver in male Wistar laboratory rats. 24 male Wistar laboratory rats were divided into three groups. The control group included rats which received 0.5 ml of physiological saline. The second and third groups received 0.5 ml of Fe2O4Zn Nano fluid at concentration levels of 100 and 200 ppm for 7 consecutive days. The rats were phlebotomized on days 2, 7, and 14 following the treatment. Serum concentration levels of liver enzymes were measured. The livers were removed from the bodies on day 14. Serum glutamic oxaloacetic transaminase (SGOT), serume glutamic-pyruvic transaminase (SGPT), and alkaline phosphatase (ALP) levels saw a significant increase in both treatment groups compared to the control group on days 2, 7, and 14. Lactate dehydrogenase (LDH) levels, however, showed a reduction in the treatment groups compared to the control group which was not statistically significant. The histopathological analysis of liver tissues in treatment groups pointed to intrahepatocellular lipid accumulation, mild portal inflammation, hepatocyte inflammation, hyperemia, centrilobular, intravascular and portal red blood cell (RBC) aggregation, and centrilobular necrosis. Results from the second treatment group were more drastic than the first treatment group. Results demonstrate the histopathological effects of Fe2O4Zn on liver tissues and enzyme levels in the treatment groups compared to the control group

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