This research was conducted during period of February 2016 to July 2017 to detect Toxoplasma gondii recruiting 106 female patients with repeated abortion with age between 18-43 years who attended Al-Fayeth clinical laboratory in Baghdad. The diagnosis was done by Immunochromatography and ELISA methods. Blood sample was taken from each patient as well as other 30 healthy control of same age group. The study included measurement of concentration of interleukin-8, interleukin-10, leukotriene B4 and calcium in sera of patients and control. The result indicated presence of the anti-Toxoplasma IgG level in 45 cases, and anti-Toxoplasma IgM in 76 cases out of 106 cases of women by immunochromatography methods whereas anti-Toxoplasma IgG in 40 cases and Anti-toxoplasma IgM in 42 cases by ELISA methods. Also, the result indicated increasing levels of IL-8 and IL-10 in patients’ sera and decreasing level of leukotrienes B4 and calcium compared to healthy women (control).